By developing and using a novel biosensor namely CaspaseTracker, we further discovered that reversal of apoptosis can occur at egg chambers in fruit flies after transient exposure to apoptosis-inducing environmental insults, such as protein starvation and cold shock 8, 13. These events include mitochondrial outer membrane permeabilization (MOMP) for cytochrome c release, activation of execution caspase proteases for cleaving cellular substrates, DNA damage caused by apoptotic DNases, exposure of “eat me” signal of phosphatidylserine to cell surface, cell shrinkage and plasma membrane blebbing due to the destruction of cellular structure components, and formation of apoptotic bodies that dying cells fragment into small pieces for engulfment by phagocytes or neighbouring cells 6, 7, 8, 9, 10, 11, 12, 13. Challenging this prevailing view, we have discovered that this cell suicide process can be reversible in primary cells and cell lines, even in dying cells that have experienced critical apoptotic events 6, 7, 8, 9, 10, 11, 12, 13. Programmed cell death such as apoptosis has long been assumed as a one-way process, ending in cell demise 1, 2, 3, 4, 5. Therefore, reusing or reanalysing this dataset will facilitate the future studies on the physiological, pathological, and therapeutic implications of anastasis. By comparing this dataset with the transcriptomic profiles of other anastasis models generated with different combinations of cell types and cell death inducers, investigators can identify the key regulators governing reversal of apoptosis and other reversible cell death processes. This transcriptome dataset includes the conditions of the untreated cells, cells undergoing apoptosis triggered by incubating with cell death inducer of 4.5% ethanol for 5 hours, and apoptosis reversal of ethanol-induced cells at the early (3 rd hour), middle (6 th hour), and late (24 th, 48 th hour) stages after being washed with and incubated in fresh cell culture medium. Here, we describe a comprehensive dataset containing time-course mRNA expression profiles for reversal of ethanol-induced apoptosis in mouse primary liver cells in νitro. Reversal of apoptosis is the first example of anastasis. Anastasis is a cell recovery mechanism that rescues dying cells from the brink of death.
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